Tuesday, November 23, 2010

DNA Fingerprinting

Introduction:

1. DNA is unique for everyone. The only exception is if a person has what?
If someone has an identical twin, the DNA is not unique between them.

2. What are DNA fingerprints used for?

DNA fingerprints can be used to find a biological parent or even finding criminals and suspects.


Part 1 “It Takes a Lickin”

3. What “crime” was committed?
Somebody licked Jimmy Sweet's holographic NOVA lollipop.

4. What bodily fluid was removed from the “crime scene” to get DNA?

Saliva was removed to get DNA.

Part 2 “DNA Fingerprinting at the NOVA Lab”


5. What does a restriction enzyme do?
A restriction enzyme cuts DNA at certain parts of the DNA. Where the DNA is cut depends on the codes in both DNA and the enzyme.

6. What is agarose gel?
Agarose gel is a thick, jello- like liquid that is a "molecular strainer", meaning that it will allow small pieces of DNA to move farther and more easily than the longer pieces.

7. What is electrophoresis?
Electrophoresis is the process of moving molecules with an electric current.

8. Smaller fragments of DNA move ____________ than longer strands?
Smaller fragments of DNA move more easily than longer strands.

9. Why do you need to place a nylon membrane over the gel?
You need to place a nylon membrane over the gel because the DNA will transfer to the nylon, allowing an easier representation to work with than the agarose, which is hard to work with.

10. Probes attach themselves to __________
Probes attach themselves to DNA fragments on the nylon membrane.

11. Which chemical in your "virtual lab" is radioactive? The probes are radioactive because they were labeled radioactively so that they can be traced.


12. Sketch your DNA fingerprint.

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13. Based on your DNA fingerprint, who licked the lollipop?

Honey licked the lollipop.


Click on the Link "DNA Workshop" (if this link won't load, scroll down to the bottom where it says "try the non-java script version")

Once you’re there, go to the link “DNA Workshop Activity” and practice with DNA replication and protein synthesis.

Browse the DNA Workshop site.

14. What kinds of things could you do at the DNA workshop?

You could go to the DNA Workshop activity, learn more about cell division, DNA replication and protein synthesis, and click on links to learn more about the people who first discovered these processes and the such.

Find an Article about DNA

Go to http://www.thegenesite.com/

15. Read an article about genetics at this site that you might find interesting, or use the "Search" box in the upper right hand corner to search for DNA fingerprinting.

Title of Article ____________________________ Author and Date ______________________________________

Summarize what the article was about. Write this in a paragraph format.

NOTE: This site didn't work at the time of my usage, it is just a search site that provides advertisements only.

Wednesday, November 3, 2010

Mitosis

Mitosis Tutorial:
1. Which stage does the following occur:

Chromatin condenses into chromosomes.

Prophase.

Chromosomes align in center of cell.
Metaphase.

Longest part of the cell cycle.
Anaphase.

Nuclear envelope breaks down.
Prometaphase.

Cell is cleaved into two new daughter cells.
Cytokinesis.

Daughter chromosomes arrive at the poles.
Telophase.
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2. The colored chromosomes represent chromatids. There are two of each color because one is an exact duplicate of the other.

--How many chromosomes are visible at the beginning of mitosis?
Four chromosomes are visible.

-- How many are in each daughter cell at the end of mitosis?
Four chromosomes are in each daughter cell.

--The little green T shaped things on the cell are:
Centrioles.

-- What happens to the centrioles during mitosis?
The centrioles gravitate towards the poles of the cell during mitosis, and separate the cell.

3 . Identify the stages of these cells:
The first image is of a cell in metaphase.
The second image is of a cell in cytokinesis.
The third image is of a cell in prophase.
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Another Mitosis Animation:

Prophase: The centrioles begin to go towards the cellular poles, and the chromosomes fully form. Also, they spread out.

Metaphase: In metaphase, the spindle fibers attach to the chromosomes and the chromosomes align in the center of the cell.

Telophase: In telophase, the nuclear envelope forms, and chromosomes go towards the poles of the cell, to finally split the cell into two daughter cells.
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Onion Root Tip - Online Activity


Number of Cells:
Interphase: 20
Prophase: 10
Metaphase: 3
Anaphase: 2
Telophase: 1
Total: 36
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Percent of Cells:
Interphase: 55%
Prophase: 28%
Metaphase: 8%
Anaphase: 6%
Telophase: 3%
Total: 100%
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Mitosis in Whitefish and Onion Roots

Whitefish
View 1: Telophase
View 2: Metaphase
View 3: Prophase
View 4: Anaphase

Onion
View 1: Prophase
View 2: Metaphase
View 3: Prophase
View 4: Telophase
View 5: Anaphase